Diallyl trisulfide is a major garlic-oil organosulfur constituent and one of the most studied isolated garlic sulfides in preclinical literature. The current source-grounded picture is strongest for identity, constituent occurrence, antifungal activity, and mechanistic animal or cell studies involving hydrogen-sulfide donation, Nrf2 signaling, and NF-kB-related inflammation pathways. That evidence is still preclinical, so AromaForge should present diallyl trisulfide as a highly researched garlic constituent rather than a clinically validated therapeutic ingredient.
Chemical identity and composition
PubChem lists diallyl trisulfide as C6H10S3 with IUPAC name 3-(prop-2-enyltrisulfanyl)prop-1-ene. In the local archive's China garlic oil record, diallyl trisulfide appears at 21.98%, and a 2021 garlic-oil paper reported it as the top constituent at 39.79% in the tested sample.
Aroma and practical use
- Use the recorded garlic, pungent, sulfury, penetrating, sulfurous descriptors to explore a blend's scent character; perceived strength also depends on the other ingredients.
- Review the flagged use concerns across every contributing oil and the selected dilution.
- Read the route, preparation and experimental context before using a study finding to choose an oil.
Reported benefits and effects
Findings retain their study context. An animal or laboratory result describes that experiment.
wood rot fungi antifungal activity
A full-text Molecules study reported that garlic oil contained 39.79% diallyl trisulfide and that isolated diallyl trisulfide was the most effective of the three major garlic sulfides tested against two wood-rotting fungi, with IC50 values of 56.1 and 31.6 microg/mL.
Measured: IC50 and comparative activity versus other garlic sulfides
This experimental finding does not establish benefit, dose or safety in people using this blend.
ischemia reperfusion cardiac model
A PubMed-listed study reported that diallyl trisulfide increased endogenous hydrogen sulfide, improved mitochondrial coupling after reperfusion, activated eNOS, and supported cardioprotection in a mouse ischemia-reperfusion model.
Measured: H2S levels, infarct size, mitochondrial coupling, nitric oxide bioavailability
This experimental finding does not establish benefit, dose or safety in people using this blend.
acute lung injury inflammation model
A PubMed-listed mouse study reported that diallyl trisulfide affected TNF-alpha expression and NF-kB activity in lipopolysaccharide-induced acute lung injury, supporting the broader inflammation-modulation pattern seen for garlic polysulfides.
Measured: TNF-alpha expression and NF-kB activity
This experimental finding does not establish benefit, dose or safety in people using this blend.
insect fertility and energy metabolism model
A 2023 abstract reported that diallyl trisulfide impaired spermatogenesis, sperm motility, ATP content, and lipid homeostasis in an insect model, showing strong biological activity but not providing human safety data.
Measured: sperm number, sperm motility, ATP, lipid homeostasis
Apply this finding only to the studied preparation, participants, route and outcome. It does not establish the same result for a custom oil blend.
Use concerns and concentration limits
Sensitization concernFlagged
Flagged in the constituent reference; assess the source, route and finished-product concentration.
Phototoxicity concernNot flagged
Not flagged in the current model. This is not proof of absence or unrestricted safety.
Oxidation concernNot flagged
Not flagged in the current model. This is not proof of absence or unrestricted safety.
Penetration enhancementNot flagged
Not flagged in the current model. This is not proof of absence or unrestricted safety.
No numeric constituent-specific use limit is available here. Missing is not an unrestricted-use clearance.
No constituent-specific IFRA leave-on limit, phototoxic restriction, or human dermal-sensitization threshold was located in the reviewed public sources for isolated diallyl trisulfide.
This reference does not establish a therapeutic result or a safe use level for a custom oil blend.
No direct public citation is attached to this entry.
Diallyl trisulfide is strongly bioactive across multiple nonhuman models, but those findings do not establish safe or effective human therapeutic use.
This reference does not establish a therapeutic result or a safe use level for a custom oil blend.
No direct public citation is attached to this entry.
Because garlic sulfide-rich oils are generally treated as irritating and highly potent in topical practice, archive copy should avoid implying that isolated diallyl trisulfide is a mild skin ingredient.
This reference does not establish a therapeutic result or a safe use level for a custom oil blend.
No direct public citation is attached to this entry.
Constituents it may interact with
No constituent-pair interaction is recorded for this identity. The report does not assume either amplification or incompatibility.
How the effects were investigated
hydrogen sulfide donor behavior
A myocardial ischemia-reperfusion study reported that diallyl trisulfide increased circulating and myocardial hydrogen sulfide and improved post-ischemic mitochondrial and functional measures, supporting its role as a slow hydrogen-sulfide donor.
This experimental finding does not establish benefit, dose or safety in people using this blend.
keap1 cys288 linked nrf2 activation
A mechanistic PubMed-listed study reported that diallyl trisulfide may interact with Keap1 cysteine 288, helping explain constituent-induced Nrf2 activation and defensive gene upregulation.
This experimental finding does not establish benefit, dose or safety in people using this blend.
What this report cannot establish
- A reported constituent percentage does not establish an effective human therapeutic dose or the amount absorbed into the body.
- The presence of a constituent does not guarantee a clinical benefit, rash treatment or sleep improvement from a custom blend.
- An unflagged concern or missing use limit does not establish safety for ingestion, inhalation, skin exposure or a particular person.
- A candidate constituent interaction does not establish that two oils amplify one another at the selected proportions.
- Constituent descriptors alone do not establish a precise top, heart or base lifetime in a finished blend.
Known data gaps (7)
- A bounded search (2026-07-11) for diallyl trisulfide-specific dermal sensitization/contact-dermatitis case reports returned only diallyl disulfide literature (the better-documented garlic allergen); no trisulfide-specific human sensitization, IFRA, or dermal-limit source was located, so sensitizing/dermal_limit fields remain unverified rather than inferred from the disulfide analog.
- No human clinical trials specific to isolated diallyl trisulfide were verified in this pass.
- No constituent-specific topical safety threshold or leave-on dermal limit was located in the reviewed public sources.
- Most mechanistic and benefit-oriented claims still rely on animal, cell, or other nonhuman models rather than translational human evidence.
- Worker c8 verification (2026-07-13): no dedicated RIFM/IFRA safety assessment for diallyl trisulfide (CAS 2050-87-5) was located (searched specifically; only supplier/odor-profile pages and an unrelated antifungal nanoemulsion study found). Sensitizing:true is set based on peer-reviewed garlic allergen literature identifying mono-, di-, and trisulfides (including diallyl trisulfide) as contributors to allergic contact dermatitis to garlic, by analogy with the closely related and RIFM-assessed diallyl disulfide. No verified numeric dermal limit percentage located; prior 0.3% placeholder corrected to null.
- No constituent-specific numeric dermal reference is available here.
- Some findings do not record an explicit exposure route; consult the linked study before applying them to a use.